Environment directorate, organisation for economic cooperation and development, paris, france. Thirdly, the facilitys biosafety manual, which describes the biosafety rules and operations within the nuitm bsl3 laboratory, is discussed in detail. Its primary focus should be to provide pertinent information to help execute the operations of the lab in a safe and professional manner. Footnote cen workshop 31 laboratory biosafety and biosecurity. Biosafety in microbiological and biomedical laboratories cdc. Pis should use the information in this manual to develop site specific safety procedures for their laboratory. For this new edition, therefore, the manual has been. Oecd series on principles of good laboratory practice and compliance monitoring, number 1. Oct 03, 2014 daily cleaning, disinfection, replacement of laboratory consumables and changing prefilters and hepafilters are described in detail. Bmbl 5th edition, rev 122009, section iv laboratory biosafety level criteria. Biosafety and biosecurity center for global health science. This manual does not address issues of chemical or radiation safety.
It is recommended that countries without appropriate biosafety capacity to perform a laboratory diagnosis on cases under investigation suspected and probable should send specimens to a designated who. The manual now covers risk assessment and safe use of recombinant dna technology, and provides guidelines for the comissioning and. The lsc develops policies and guidelines to assist in addressing hazardous materials and processes in the laboratory workplace to ensure. Biosafety in microbiological and biomedical laboratories 5th ed. Tuberculosis laboratory biosafety manual ncbi bookshelf. General and specific biosafety guidelines for handling sarscov2 specimens are provided below. The classification of emerging viruses is therefore based on two measures. Chapman university biosafety manual 3 while not a regulation, the bmbl has been revised several times and is commonly seen as the gold standard for biosafety. This manual is applicable to all laboratory, research, service and support activities that may involve exposure to biohazardous agents or materials and that may come under the oversight of the institutional biosafety committee ibc. The manual encouraged countries to accept and implement basic concepts in biological safety and to. The following practices incorporate minimal practices and provide guidance for ensuring the protection of personnel, research, and the environment for. Laboratory biosafety manual world health organization.
Chapter 1620 canadian biosafety handbook, second edition. The who laboratory biosafety manual 4th edition youtube. Chapman university is using the bmbl fifth edition, 2009 as the basis for this biosafety manual. For this new edition, the manual has been extensively revised and expanded. Recognizing the biosafety levels for cne this quick learn lesson will take approximately 15 minutes to complete.
The goals of the biological safety program, referenced in this manual as the biosafety program, are to protect laboratory workers, the public, and the environment from potentially hazardous biological agents. For more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in. Laboratory biosafety for handling emerging viruses. Laboratories need leaders who can effectively utilize the laboratories resources, maximize the laboratoriescapacity to detect disease, and advocate for laboratories in a fluctuating health care environment. The laboratory biosafety manual should be a living, working document that is an important resource for personnel engaged in the activities using biological and or rdna materials. The princeton university biosafety manual is intended to be a resource for information, guidelines, policies, and procedures that will enable and encourage those working in the laboratory environment to work safely and reduce or eliminate the potential for exposure to biological hazards. Its goals are to provide a professional association that represents the interests and needs of practitioners of biological safety, and to provide a forum for the continued and timely exchange. Who extended biosafety advisory group bag meetings, geneva, 2425 november 2014, and 15 december 2016 key recommendation 2014 revision to the who biosafety manual is both a necessary and a priority key recommendation 2016 general agreement for the proposed modifications to the manual, the revision of which remains a priority. Who laboratory biosafety manual lbm revision update. These guidelines for biosafety laboratory competency outline the essential skills, knowledge, and abilities required for working with biologic agents at the three highest biosafety levels bsls levels 2, 3, and 4. Aug 06, 2010 laboratory biosafety manual third edition world health organization geneva 2004 slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Receive training in standard microbiological techniques.
Biosafety manual office of environmental health and safety. When you have completed this lesson, you will be able to identify characteristics of the four biological safety levels. National guidelines on laboratory biosafety should be followed under all circumstances. All laboratory personnel shall engage in good microbiological laboratory practices at all times.
After attending this session, participants will be able to differentiate the 3rd and 4th edition of the who laboratory biosafety manual. Laboratory guidelines research institute for tropical medicine. Visitors have to be accompanied with someone allowed to enter and be briefed on how to behave within a laboratory. Though the 4th edition is in draft form the time to. California occupational safety and health administration calosha. Absa international absa was founded in 1984 to promote biosafety as a scientific discipline and serve the growing needs of biosafety professionals throughout the world. Who laboratory biosafety manual updates absa conference. I have received, in the laboratory, safety training on the biological materials listed below. Sample laboratory biosafety manual for microbiological activities. Who guidance on regulations for the transport of infectious substances, 20 2014 who hsegcr2012. The laboratory safety committee lsc laboratory safety committee at bu provides guidance and administrative oversight of environmental health and safety in bus academic research and teaching laboratories. Biosafety level refers to the stringency of biocontainment precautions deemed necessary by the centers for disease control and prevention cdc for laboratory work with infectious materials. This biosafety manual is applicable to all laboratory, research, teaching, service, and support activities that may involveexposure to biohazard agents or materials ous or otherwisecomes under the purview of the institutional biosafety committee ibc at the university of montana. A laboratory acquired infection is known or suspected, or b.
Major revisions to the 2014 institutional laboratory biosafety manual. Manual on collection, storage, and transport of specimens for tb testing producing reliable test results does not rely solely on the right performance of the stepbystep procedures of the test itself. Such specimens should be labeled accordingly, and the laboratory should be alerted to ensure proper specimen handling. Laboratory biosafety manual were published in 1993 and 2004 respectively 2,3. For additional information on handling sarscov2 specimens, refer to the laboratory biosafety frequently asked questions. This manual is a helpful reference and guide for establishing national codes of practice for the safe handling of pathogenic microorganisms in laboratories. Relation of risk groups to biosafety levels, practices and equipment risk group biosafety level laboratory type laboratory practices safety equipment 1 basic biosafety level 1 basic.
Guidelines for biosafety and laboratory competency. A spill of any quantity involving an agent infectious to humans, plants, or animals occurs in a public area. In addition, bsl3 practices and procedures are required for largescale culture of bloodborne pathogens. Stop tb partnership global laboratory initiative gli. Activities that are specifically addressed are those involving. The third edition of the who laboratory biosafety manual is a helpful reference and guide to nations that accept the challenge to develop and establish national codes of practice for securing microbiological assets, yet ensuring their availability for clinical. The ibc advocates the use of biosafety precautions that effectively reduce or. For more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. If you continue browsing the site, you agree to the use of cookies on this website. I have read and understand the georgia tech biosafety manual, and will abide by the policies set forth in that document.
Who laboratory biosafety manual having served for global biosafety community hereinafter, who manual for more than 20 30 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. Bsl3 laboratory user training program at nuitmkemri. For biosafety assessment, the main consideration is the ability of viruses to cause local or widespread disease from laboratory accidents. The development of this fourth edition of the laboratory biosafety manual has. Ventilated workstation manual for afb smear microscopy. Biosafety level 4 bsl4 is the highest level of biosafety precautions, and is appropriate for work with agents that could easily be aerosoltransmitted within the laboratory and cause severe to fatal disease in humans for which there are no available vaccines or treatments.
Please go to the table of contents page to access the entire publication total 178 p. Biosafety manual approved july 28, 2014 page 3 of 17 tuberculosis, st. It all starts in the acceptance of correct, adequate and quality specimens brought to the laboratory. Good microbiological technique and appropriate use of biosafety equipment by well trained staff remain the fundamental elements of laboratory biosafety. Laboratory biosafety is the process of applying a combination of administrative controls, containment principles, practices and procedures, safety equipment, emergency preparedness, and facilities to enable laboratory staff to work safely with potentially infectious microorganisms. Oecd principles in good laboratory practice as revised in 1997.
1642 598 65 14 540 547 1345 614 1116 1175 1156 10 292 1357 1195 799 1351 831 411 37 554 151 659 931 865 820 84 787 419 775 1017 512 702 180 748 871